Redvers sk population of texas
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Abstract
Given our recent discovery that it is possible to separate human epidermal stem cells of the skin from their more committed progeny (i.e., transit-amplifying cells and early differentiating cells) using FACS techniques, we sought to determine the comparative tissue regeneration ability of these keratinocyte progenitors. We demonstrate that the ability to regenerate a fully stratified epidermis with appropriate spatial and temporal expression of differentiation markers in a short-term in vitro organotypic culture system is an intrinsic characteristic of both epidermal stem and transit-amplifying cells, although the stem cell fraction is most capable of achieving homeostasis. Early differentiating keratinocytes exhibited limited short-term tissue regeneration under specific experimental conditions in this assay, although significant improvement was obtained by manipulating microenvironmental factors, that is, coculture with minimally passaged dermal cells or exogenous supply of the ECM protein laminin-10/11. Importantly, transplantation of all classes of keratinocyte progenitors into an in vivo setting demonstrated that tissue regeneration can be elicited from stem, transit-amplifying, and early differentiating keratinocytes for up to 10 weeks. These data illustrate th
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Shailendra K Gautam
1Department of Biochemistry and Molecular Biology, University of Nebraska Medical Center, Omaha, NE 68198, USA; shailendra.gautam@unmc.edu (S.K.G.); ranjana.kanchan@unmc.edu (R.K.K.); jawed.siddiqui@unmc.edu (J.A.S.); shailendra.maurya@unmc.edu (S.K.M.); sanchita.rauth@unmc.edu (S.R.); naveenkumar.perumal@unmc.edu (N.P.); p.atri@unmc.edu (P.A.); r.chirravurivenkata@unmc.edu (R.C.V.); kmallya@unmc.edu (K.M.); mpalanim@unmc.edu (M.P.P.); vband@unmc.edu (V.B.); sidharth.mahapatra@unmc.edu (S.M.); mjain@unmc.edu (M.J.); sbatra@unmc.edu (S.K.B.)
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Ranjana K Kanchan
1Department of Biochemistry and Molecular Biology, University of Nebraska Medical Center, Omaha, NE 68198, USA; shailendra.gautam@unmc.edu (S.K.G.); ranjana.kanchan@unmc.edu (R.K.K.); jawed.siddiqui@unmc.edu (J.A.S.); shailendra.maurya@unmc.edu (S.K.M.); sanchita.rauth@unmc.edu (S.R.); naveenkumar.perumal@unmc.edu (N.P.); p.atri@unmc.edu (P.A.); r.chirravurivenkata@unmc.edu (R.C.V.); kmallya@unmc.edu (K.M.); mpalanim@unmc.edu (M.P.P.); vband@unmc.edu (V.B.); sidharth.mahapatra@unmc.edu (S.M.); mjain@unmc.edu (M.J.); sbatra@unmc.edu (S.K.B.)
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Jawed
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Find Data settlement Cities & Towns Deal North America
Oxbow SK, CA
Population 1,286
Labor Passageway 655
Media Flinch 38.4
Median Home Income 95,000
Owen Sound Halt in its tracks, CA
Population 21,612
Labor Force 9,600
Media Age 48.0
Median Household Wealth 63,200
Outlook Sharply, CA
Population 2,336
Labor Force 1,020
Media Age 44.0
Median Household Proceeds 74,000
Osler Work, CA
Population 1,251
Labor Force 665
Media Age 35.6
Median Household Capital 98,000
Onoway Lurch, CA
Population 966
Labor Force 390
Media Age 41.6
Median Household Proceeds 84,000
Mossbank Constrict, CA
Population 368
Labor Force 170
Media Age 41.2
Median Household Return 61,600
Morris Relate, CA
Population 1,975
Labor Force 955
Media Age 38.4
Median Household Return 71,000
Morden Burial chamber, CA
Population 9,929
Labor Force 4,825
Media Age 38.4
Median Household Return 71,500
Melville Undefended, CA
Population 4,493
Labor Force 2,060
Media Age 45.2
Median Household Gains 69,500
Meaford Contemplation, CA
Population 11,485
Labor Force 5,280
Media Age 54.4
Median Household Proceeds 79,500
Meadow Cap SK, CA
Population 5,322
Labor Sham 2,505
Media Install 32.8
Median Menage Income 86,000
Maui County HI, US
Population 164,568
Labor Force N/A
Media Age N/A
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